Up-regulated expression of CD147 gene in malignant bone tumor and the possible induction mechanism during osteoclast formation
AUTOR(ES)
Qiao, Suchi, Liu, Chang, Xu, Weijie, AZhaTi, WuBuLi, Li, Cheng, Wang, Zhiwei
FONTE
Braz J Med Biol Res
DATA DE PUBLICAÇÃO
23/07/2018
RESUMO
It is increasingly evident that the microenvironment of bone can influence cancer phenotype in many ways that favor growth in bone. CD147, a transmembrane protein of the immunoglobulin (Ig) superfamily, was identified independently in different species and has many designations across different species. However, expression levels of CD147 mRNA in bone cancer have not been described. In this study, we have used real-time fluorescence quantification (RT-PCR) to demonstrate CD147 expression in malignant bone cancer and benign bone tumor tissues. The results suggested that the expression of CD147 gene was significantly up-regulated in malignant bone cancer. Moreover, we found that over-expressed RANKL progressively enhanced osteoclast formation up to 48 h, which suggested that RANKL could promote the formation of osteoclast, indicating that both CD147 and RANKL play important roles in the formation of osteoclasts. Furthermore, the expressions of four osteoclast specific expression genes, including TRACP, MMP-2, MMP-9 and c-Src, were analyzed using RT-PCR. The results indicated that four osteoclast-specific expression genes were detectable in all osteoclast with different treatments. However, the highest expression level of these four osteoclast-specific expression genes appears in the CD147+ RANKL group and the lowest expression level of these four osteoclast-specific expression genes appears with si-RANKL treatment. Characterization of the role of CD147 in the development of tumors should lead to a better understanding of the changes occurring at the molecular level during the development and progression of primary human bone cancer.
Documentos Relacionados
- ACE2 gene expression is up-regulated in the human failing heart
- A Plant Gene Up-Regulated at Rust Infection Sites
- Senescence-specific gene expression fingerprints reveal cell-type-dependent physical clustering of up-regulated chromosomal loci
- Aromatase (Cyp19) expression is up-regulated by targeted disruption of Dax1
- Zf9, a Kruppel-like transcription factor up-regulated in vivo during early hepatic fibrosis