Uma segunda familia de proteinas indutoras de necrose encontrada em Moniliophthora perniciosa, causador da doença Vassoura-de-Bruxa em cacaueiros / A seconda family of necrosis inducing protein found in Moniliophthora perniciosa the casual agent of witche´s broom disease in cacao

AUTOR(ES)

Gustavo Henrique Alcala Zaparoli

DATA DE PUBLICAÇÃO

2007

RESUMO

The hemibiotrophic basidiomycete Moniliophthora perniciosa is the causal agent of the Witches’ Broom Disease of cacao. The infection in pod is characterized by the formation of hypertrophic and hyperplasic tissues in the biotrophic phase, forming the green broom, which is followed by necrosis and complete degradation of the organ, causing the dry broom. We found in the fungus genome at least five sequences encoding putative proteins similar to cerato-platanin-like proteins, a class of proteins initially found in the phytopathogen Ceratocystis fimbriata. One M. perniciosa cerato-platanin gene (MpCP1) was expressed “in vitro” and proved to have necrosis inducing ability in tobacco and cacao leaves. The protein is present in solution as dimers and is able to recover necrosis activity after heat treatment. Transcription analysis “ex planta” showed that MpCP1 is more expressed in biotrophic-like mycelia in comparison to saprotrophic mycelia. The necrosis profile presented is different from that caused by MpNEPs (other family of elicitors also found in M. perniciosa). Most remarkable, mixture of MpCP1 with MpNEP2 led to a synergic necrosis effect very similar to that found in naturally infected plants.

ASSUNTO(S)

moniliophthora perniciosa cerato-platanina elicitors elicitores cerato-platanin moniliophthora perniciosa

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