Um enfoque multigênico para a genealogia comparada de Betacoronavirus em bovinos e equinos / A multigene approach for a compared genealogy of Betacoronavirus from cattle and horses

AUTOR(ES)

Iracema Nunes de Barros

FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

21/01/2011

RESUMO

Gastroenteritis is one of the leading causes of morbidity and mortality amongst young and newborn animals, often caused by multiple intestinal infections, being rotavirus and Bovine coronavirus (BCoV) the main viral causes in cattle. BCoV has a worldwide distribution and caused diarrhea in calves, winter dysentery in adult cattle and respiratory disease, while in horses coronaviruses lead to neonatal enterocolitis in foals. Taking into account that BCoV is more largely studied than equine coronaviruses and the possibility of interspecies transmission of these viruses, this research aimed to assess a multigenic comparison of coronaviruses from adult cattle with winter dysentery and calves with neonatal diarrhea as well as from equines, all from Brazil, based on partial sequences of the hemagglutinin-esterase (HE), spike (S) and nucleoprotein (N) genes. To this end, 11 samples from dairy cows with winter dysentery and 27 from horses were tested for Betacoronavirus using an RT-PCR targeted to the RdRp gene and the positive samples were next submitted to RT-PCRs to the partial amplification and sequencing of N, HE and S genes for genealogic analysis. Besides, 15 calves samples previously studied for the same S gene region were also submitted to the N and HE genes RT-PCRs and sequencing for genealogic analysis. Sequences representative of the population under study were obtained for all genes. It could be concluded that enteric BCoV genealogy from newborn and adult cattle is directly associated to geographic patterns when S and HE genes are taken into account, with a less-resolved genealogy for the HE and N genes, with a trend for an age-related segregation pattern for the last and also that horses might present Betacoronavirus undistinguishable from those found in cattle, a fact previously unknown.

ASSUNTO(S)

bovine bovinos coronavirus coronavírus epidemiologia equine equinos genealogia genealogy molecular epidemiology

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