Ultrasound-Assisted Saponification Coupled with Gas Chromatography-Flame Ionization Detection for the Determination of Phytosterols from Passion Fruit Seed Oil
AUTOR(ES)
Rotta, Eliza M., Silva, Michele C. da, Maldaner, Liane, Visentainer, Jesuí V.
FONTE
J. Braz. Chem. Soc.
DATA DE PUBLICAÇÃO
2018-03
RESUMO
An ultrasound-assisted saponification (UAS) method for the determination of phytosterols (campesterol, β-sitosterol and stigmasterol) in passion fruit seed oil, followed by gas chromatography with flame ionization detection, was developed and validated. Under the optimum conditions, good linearity with correlation coefficients higher than 0.99 was obtained for all analytes. The recoveries for the proposed approach were between 103 and 107%, the limits of quantification were between 70 and 180 μg kg-1 and the precision values expressed in terms of relative standard deviations (RSD) were lower than 19%. The developed method was successfully applied to the analysis of passion fruit seed oil samples from different species, including yellow passion fruit, sweet granadilla and sweet passion fruit. In all species of analyzed passion fruit seed oils, β-sitosterol was found with the highest abundance, ranging from 0.90 ± 0.02 to 1.60 ± 0.01 mg g-1 of oil, followed by stigmasterol (0.70 ± 0.01 to 1.40 ± 0.12 mg g-1 of oil) and campesterol (0.10 ± 0.01 to 0.30 ± 0.06 mg g-1 of oil). Furthermore, the developed method proved to be a time-saving and efficient analytical procedure for the determination of phytosterols in oil samples.
Documentos Relacionados
- ULTRASOUND-ASSISTED TRANSESTERIFICATION OF SOYBEAN OIL USING COMBI-LIPASE BIOCATALYSTS
- Ultrasound-assisted surfactant-enhanced emulsification microextraction combined with hplc for the determination of estrogens in water
- Ultrasound-assisted treatment of coconut water samples for potentiometric stripping determination of zinc
- Aqueous Ultrasound-Assisted Extraction for the Determination of Fluoroquinolones in Mangrove Sediment by High-Performance Liquid Chromatography and Fluorescence Detector
- Ultrasound-assisted emulsification solidified floating organic drop microextraction for the determination of trace cadmium in water samples by flame atomic absorption spectrometry