Typing class I HLA-A gene using a nested PCR-RFLP procedure
AUTOR(ES)
Castelli, E.C., Gil, D.S., Veiga, L.C.S., de Camargo, J.L.V.
FONTE
Brazilian Journal of Medical and Biological Research
DATA DE PUBLICAÇÃO
2005-06
RESUMO
In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.
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