Type C RNA virus-specific antibody in human systemic lupus erythematosus demonstrated by enzymoimmunoassay.
AUTOR(ES)
Mellors, R C
RESUMO
Postmortem study of proliferative glomerulonephritis associated with human systemic lumpus has previously shown that an antigen related to mammalian type C RNA viral core (p30) proteins is deposited in the renal glomerular lesions with human immunoglobulins in an immune-complex pattern. In the present work, human immunoglobulins were sequentially eluted from the lupus glomerular immune deposits and were assayed by a sensitive enzymoimmunoassay developed for the measurement of anti-p30 antibody activity against purified viral p30 proteins of mammalian type C viruses. Human immunoglobulins showing specific anti-p30 antibody activity, particularly against p30 antigen of feline endogenous virus RD-114 and to a smaller extent against p30 antigen of murine type C virus, were eluted by acid buffer from the glomerular immune deposits in two patients with lupus proliferative glomerulonephritis who have deposits of viral p30-related antigen in the same tissue lesions. This study adds support for the hypothesis that expression of type C viral antigen may be involved in the multifactorial pathogenesis of proliferative glomerulonephritis associated with human systemic lupus.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=392574Documentos Relacionados
- Induction of Type-C RNA Virus by Cycloheximide: Increased Expression of Virus-Specific RNA
- Chemical transformation of rat cells infected with xenotropic type-C RNA virus and its suppression by virus-specific antiserum.
- Virus-Specific RNA Synthesis in Cells Infected by Infectious Pancreatic Necrosis Virus
- Virus-Specific Cytotoxic T Lymphocytes in Human Immunodeficiency Virus Type 1-Infected Chimpanzees
- Virus-Specific mRNA Capping Enzyme Encoded by Hepatitis E Virus