Type 1 pilus-mediated bacterial invasion of bladder epithelial cells
AUTOR(ES)
Martinez, Juan J.
FONTE
Oxford University Press
RESUMO
Most strains of uropathogenic Escherichia coli (UPEC) encode filamentous adhesive organelles called type 1 pili. We have determined that the type 1 pilus adhesin, FimH, mediates not only bacterial adherence, but also invasion of human bladder epithelial cells. In contrast, adherence mediated by another pilus adhesin, PapG, did not initiate bacterial internalization. FimH-mediated invasion required localized host actin reorganization, phosphoinositide 3-kinase (PI 3-kinase) activation and host protein tyrosine phosphorylation, but not activation of Src-family tyrosine kinases. Phosphorylation of focal adhesin kinase (FAK) at Tyr397 and the formation of complexes between FAK and PI 3-kinase and between α-actinin and vinculin were found to correlate with type 1 pilus-mediated bacterial invasion. Inhibitors that prevented bacterial invasion also blocked the formation of these complexes. Our results demonstrate that UPEC strains are not strictly extracellular pathogens and that the type 1 pilus adhesin FimH can directly trigger host cell signaling cascades that lead to bacterial internalization.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=203355Documentos Relacionados
- Pilus-mediated adherence of Escherichia coli K1 to human oral epithelial cells.
- The Shufflon of Salmonella enterica Serovar Typhi Regulates Type IVB Pilus-Mediated Bacterial Self-Association
- Inhibition of pilus-mediated adhesion of Pseudomonas aeruginosa to human buccal epithelial cells by monoclonal antibodies directed against pili.
- Pilus-Mediated Adherence of Haemophilus influenzae to Human Respiratory Mucins
- The meningococcal PilT protein is required for induction of intimate attachment to epithelial cells following pilus-mediated adhesion