Two interferon mRNAs in human fibroblasts: in vitro translation and Escherichia coli cloning studies.
AUTOR(ES)
Weissenbach, J
RESUMO
Two mRNA species that produce biologically active interferon were isolated from human fibroblasts and studied by size fractionation and cloning in Escherichia coli plasmid pBR322. The major fibroblast interferon (Hu IFN-beta 1) is coded for by the smaller of the two mRNAs, an 11S species, 900 nucleotides long, which in cell-free systems yields a 20,000 Mr protein. The second interferon mRNA species (Hu IFN-beta 2) is 14S, about 1300 nucleotides long, and codes for another protein of 23,000-26,000 Mr. The two interferon mRNAs do not cross-hybridize. Both are induced by poly(rI.rC), but IFN-beta 2 mRNA is induced to about 10% in cells by cycloheximide treatment alone whereas under these conditions IFN-beta 1 is not induced.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=350459Documentos Relacionados
- Altered Cellular mRNA Levels in Human Cytomegalovirus-Infected Fibroblasts: Viral Block to the Accumulation of Antiviral mRNAs
- Translation inhibitors stabilize Escherichia coli mRNAs independently of ribosome protection
- Magnesium-dependence of in vitro translation programmed by gene-specific mRNAs
- In Vitro Translation of Autographa californica Nuclear Polyhedrosis Virus Early and Late mRNAs
- Detection of two growth hormone receptor mRNAs and primary translation products in the mouse.