Two-dimensional gel analysis of rat liver nuclear proteins after thyroidectomy and thyroid hormone treatment.
AUTOR(ES)
Nikodem, V M
RESUMO
The composition of nucleoproteins in normal rat liver, in livers from thyroidectomized animals, and in thyroidectomized animals treated with 3,3',5-triiodo-L-thyronine was examined by two-dimensional gel electrophoresis. A computer program was designed to measure the relative concentrations of each protein. Approximately 500 protein subunits could be distinguished. Of these, 102 either disappeared or were markedly decreased after thyroidectomy. Shortly after administration of the thyronine (6 hr), 13 proteins reappeared and, by 24 hr, 67 additional proteins had reappeared. These changes in protein concentrations are both the greatest in number and have the largest reported as an effect of thyroid hormone. Twenty-four hr after triiodothyronine administration, some 22 proteins seen in normal liver could not be found. Two new proteins appeared in thyroidectomized rat liver nuclei. We conclude that a major and rapid effect of thyroid hormone is exerted on hepatic nucleoproteins.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=319800Documentos Relacionados
- Comparison of Nucleolar Proteins of Normal Rat Liver and Novikoff Hepatoma Ascites Cells by Two-Dimensional Polyacrylamide Gel Electrophoresis
- Two-dimensional gel electrophoresis and immunoblotting of Campylobacter pylori proteins.
- Two-dimensional gel electrophoresis and immunoblotting of Campylobacter outer membrane proteins.
- Genetic Variation in Proteins: Comparison of One-Dimensional and Two-Dimensional Gel Electrophoresis
- Two-dimensional electrophoretic analysis of encephalomyocarditis viral proteins.
