Triple-helix formation in the antiparallel binding motif of oligodeoxynucleotides containing N9- and N7-2-aminopurine deoxynucleosides
AUTOR(ES)
Parel, Serge P.
FONTE
Oxford University Press
RESUMO
Triplex-forming oligodeoxynucleotide 15mers, designed to bind in the antiparallel triple-helical binding motif, containing single substitutions (Z) of the four isomeric αN7-, βN7-, αN9- and βN9-2-aminopurine (ap)-deoxyribonucleosides were prepared. Their association with double-stranded DNA targets containing all four natural base pairs (X-Y) opposite the aminopurine residues was determined by quantitative DNase I footprint titration in the absence of monovalent metal cations. The corresponding association constants were found to be in a rather narrow range between 1.0 × 106 and 1.3 × 108 M–1. The following relative order in Z × X-Y base-triple stabilities was found: Z = αN7ap: T-A > A-T> C-G ∼ G-C; Z = βN7ap: A-T > C-G > G-C > T-A; Z = αN9ap: A-T = G-C > T-A > C-G; and Z = βN9ap: G-C > A-T > C-G > T-A.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=55712Documentos Relacionados
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