Transport of Octanoate by Pseudomonas oleovorans
AUTOR(ES)
Toscano, William A.
RESUMO
The properties of a system for octanoate transport in Pseudomonas oleovarans are described. Transport is inducible and energy dependent, shows saturation kinetics, and concentrates against a gradient. Optimal transport is at pH 6.0 and 28 C. Apparent Km and Vmax values are, respectively, 7.0 μM octanoate and 0.68 nmol of octanoate transported per min per mg (dry mass) of cells. Fatty acids from C7 to C12 are competitive inhibitors, whereas alkanes, alkenes, and esters of the same carbon chain lengths show no inhibition. The Ki values for heptanoate, nonanoate, decanoate, undecanoate, and dodecanoate are 17, 3.4, 3.2, 1.2, and 2.4 μM, respectively. The molecular specificity of this transport system is a linear hydrocarbon chain of no less than 6 to at least 11 carbon atoms and a carboxyl group.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=285416Documentos Relacionados
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