Transplante autólogo de tecido ovariano para o peritônio de ratas após congelamento e descongelamento

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

One of the major problem against reproductive medicine is the incapacity of oocyte preservation. In these cases, ovary preservation could restore hormonal e fertility capacity. Objective: Assess the ovarian function after removal of the ovaries, cryopreservation and intraperitoneal autotransplantation. Design: Prospective experimental study involving 67 rats. Materials/Methods: A total of 67 Holtzman rats were randomized to one of the three groups: Group 1 (n 5 20) bilateral oopherectomy, Group 2 (n 5 24) shame operation, Group 3 (n 5 23) cryopreserved ovarian autotransplantation group. The rats in Group 3 had frozen/thawed ovaries replaced to the peritoneum 10 days after freezing with DMSO. Daily vaginal cytology was performed for 10 days starting 7 days after surgery in group 1 and 2, and 15 days after surgery in group 3. Histologic examination of the transplanted frozen/thawed ovarian tissue was performed in all cycling animals 40 days following transplantation. Results: In group 1 none of the animal showed cytologic alterations compatible with estrus. All animals in group 2 were considered as having hormone secretion. Cytologic examination of vaginal smears from group 3 revealed 13 animals with the normal cycle sequence and 8 animals showing alterations compatible with estrus. One animal had 4 days of diestrus and 2 days of metaestrus and another had 3 days of estrus followed by one day of metaestrus and one day of distrus. Morphologic study of the ovaries showed no ischemic or inflammatory alterations and all ovaries showed follicles in different stages of maturation, i.e., viable primordial, antral follicles and corpus luteum. Conclusions: Our study demonstrates that frozen/thawed ovaries transplanted to the peritoneum without vascular anastomosis in rats preserve endocrine and reproductive functions. These findings might encourage the development of human autologous ovarian transplantation protocols for women at increased risk of ovarian failure.

ASSUNTO(S)

ovário/transplante decs medicina reprodutiva decs transplante autólogo decs oócitos decs ratos decs criopreservação decs

ACESSO AO ARTIGO

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