Transferência intratubárica videolaparoscópica de embriões ovinos fertilizados in vitro / Embryo transfer in oviduc of ovine in vitro fertilized by laparoscopy

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

The development of the technique of embryo transfer in oviduct through video-assisted laparoscopy and the demonstration of productive association between laparoscopic follicle aspiration (LOPU) and embryonic transfer laparoscopy and / or open surgery (video-assisted laparotomy)both in oviduct was the goal of the work. From the 53 animals used in the study, follicular puncture was used to obtain oocytes from 9 adult ewes for super-ovulated with hormonally stimulation, referred to as the donors. The oocytes were obtained through repeated LOPU with minimum intervals of one month from the same donor. The retrieved oocytes were matured, fertilized with frozen semen and cultured in vitro for up to two days after IVF. The cleaved embryos were transferred on the second day after IVF into synchronized receptor ewes. The transfer of embryos was first done in 10 receptors through laparoscopy and then in 34 receptors through video-assisted laparotomy, the latter being divided into two chronological stages. For the development of ET by video-assisted laparoscopy, a 5mm trocar was initially introduced ventral midline near the mammary gland for passage of the laparoscope. A second portal, in a craniolateral position was used for passage of another 5 mm trocar for introduction of forceps to manipulate the ovary. After confirming the existence of CL in one of the ovaries, a trocar was inserted on the opposite side for passage of the Foley catheter, to assist in the exposure and opening of the fimbria. A urinary catheter, containing the embryo, was then introduced into the initial portion of the oviduct for deposition of the embryos. There were 8 fetuses resulting from the transfer by laparoscopy (10 receptors), 10 fetuses resulting from the first stage of the transfer by laparotomy (14 receptors) and 30 fetuses resulting from the second stage of the laparotomy (20 receptors). The average number of fetuses per session of LOPU was 1 for laparoscopic transfer, 1.3 for the first stage of the transfer by laparotomy and 3.3 in the second stage of laparotomy. The technique of embryo transfer through video-assisted laparoscopy with cleaved embryos in oviduct was thus shown to a positive fetuss result of production, and will be promising. When associated with LOPU, the transfer of embryos in oviduct by laparoscopy / video-assisted laparotomy presented better results then other authors when compare intrauterine IVF embryo transfer.

ASSUNTO(S)

embrião ovinos sheep laparoscopia oviduct laparoscopy embryo oviduto in vitro in vitro

ACESSO AO ARTIGO

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