Transcriptional regulation of Salmonella virulence: a PhoQ periplasmic domain mutation results in increased net phosphotransfer to PhoP.

AUTOR(ES)

Gunn, J S

RESUMO

A mutation in the phoP/phoQ locus (pho-24) that results in unregulated expression of PhoP-activated genes (phenotype PhoP constitutive [PhoP(c)]) was mapped to phoQ. Change of a Thr to Ile at position 48 of PhoQ was responsible for the PhoP(c) phenotype (attenuation of mouse virulence, defects in epithelial cell invasion, and macrophage spacious phagosome formation). PhoP phosphorylation by membrane extracts required PhoQ, and PhoQ Ile-48-containing membranes demonstrated increased net phosphorylation of PhoP.

Documentos Relacionados

• A two-component regulatory system (phoP phoQ) controls Salmonella typhimurium virulence.
• Salmonella typhimurium phoP virulence gene is a transcriptional regulator.
• Constitutive Mutations of the Salmonella enterica Serovar Typhimurium Transcriptional Virulence Regulator phoP
• Regulation of nonspecific acid phosphatase in Salmonella: phoN and phoP genes.
• PhoP Can Activate Its Target Genes in a PhoQ-Independent Manner