Transcription of RNA templates by T7 RNA polymerase.
AUTOR(ES)
Arnaud-Barbe, N
RESUMO
Although highly specialized, T7 RNA polymerase seems to possess a large range of DNA- and RNA-dependent properties. To study such flexibility, we determined the ability of T7 RNA polymerase to transcribe chimeric DNA-RNA and RNA templates following initiation at a double stranded DNA promoter. We have found that T7 RNA polymerase is able to initiate on RNA templates, was processive, and was able to use templates containing RNA-RNA duplexes under standard transcription conditions. Implications of remnants of such RNA-dependent activities for T7 DNA-dependent polymerase are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=147742Documentos Relacionados
- In vitro transcription through nucleosomes by T7 RNA polymerase.
- Effect of carcinogenic adducts on transcription by T7 RNA polymerase.
- Synthetic polyamines stimulate in vitro transcription by T7 RNA polymerase.
- RNA template-directed RNA synthesis by T7 RNA polymerase.
- Stereospecificity of nucleases towards phosphorothioate-substituted RNA: stereochemistry of transcription by T7 RNA polymerase.
