Transcription initiation complexes and upstream activation with RNA polymerase II lacking the C-terminal domain of the largest subunit.

AUTOR(ES)

Buratowski, S

RESUMO

RNA polymerase II assembles with other factors on the adenovirus type 2 major late promoter to generate pairs of transcription initiation complexes resolvable by nondenaturing gel electrophoresis. The pairing of the complexes is caused by the presence or absence of the C-terminal domain of the largest subunit. This domain is not required for transcription stimulation by the major late transcription factor in vitro.

Documentos Relacionados

• The C-terminal domain of the largest subunit of RNA polymerase II and transcription initiation.
• Unusual C-terminal domain of the largest subunit of RNA polymerase II of Crithidia fasciculata.
• A protein kinase that phosphorylates the C-terminal repeat domain of the largest subunit of RNA polymerase II.
• The C-terminal repeat domain of RNA polymerase II largest subunit is essential in vivo but is not required for accurate transcription initiation in vitro.
• The C-terminal domain of the largest subunit of RNA polymerase II interacts with a novel set of serine/arginine-rich proteins.