Total volume culture technique for the isolation of microorganisms from continuous ambulatory peritoneal dialysis patients with peritonitis.

AUTOR(ES)

Dawson, M S

RESUMO

A total volume method of culturing dialysis fluid from continuous ambulatory peritoneal dialysis patients during episodes of peritonitis was developed. Concentrated culture media stored in small blood transfer bags were added directly to the drained dialysate exchange bags by the same technique used to carry out the dialysate exchange. The exchange bag with the added culture medium was incubated at 35 degrees C and observed for turbidity. Seventy-eight dialysis exchange bags from patients without clinical peritonitis (negative controls) and forty-eight dialysis exchange bags from patients with clinical peritonitis were cultured. Bacteria were recovered from all cultures of patients with clinical peritonitis (100% sensitivity) and from five cultures of negative control fluids (94% specificity). Of these isolates, 86% were gram positive, and 14% were gram negative. This technique represents an advance over previously described culture techniques in its ability to isolate the causative organism(s) in cases of peritonitis in continuous ambulatory peritoneal dialysis patients.

Documentos Relacionados

• Susceptibility testing of bacteria recovered from patients with peritonitis complicating continuous ambulatory peritoneal dialysis.
• Bordetella bronchiseptica peritonitis associated with continuous ambulatory peritoneal dialysis.
• Mycobacterium fortuitum peritonitis associated with continuous ambulatory peritoneal dialysis.
• Microbiological diagnosis of peritonitis in patients on continuous ambulatory peritoneal dialysis.
• Laboratory diagnosis of peritonitis in patients on continuous ambulatory peritoneal dialysis.