Total Synthesis of Acetate from CO2 II. Purification and Properties of Formyltetrahydrofolate Synthetase from Clostridium thermoaceticum
AUTOR(ES)
Sun, Albert Y.
RESUMO
Formyltetrahydrofolate synthetase from Clostridium thermoaceticum had optimal activity at 55 to 60 C. Km values were: adenosine triphosphate (ATP), 6.25 × 10−4m; formate, 1.67 × 10−3m; and tetrahydrofolate, 1.00 × 10−3m; at pH 8.0. There was a requirement for divalent metals and specifically for ATP.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=284899Documentos Relacionados
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