Thirty-day β-hydroxy-βmethylbutyrate supplementation with a controlled diet does not alter the morphological and morphometric characteristics of the rat diaphragm muscle

AUTOR(ES)

BUENO, Cleuber Rodrigo de Souza

FONTE

Rev. Nutr.

DATA DE PUBLICAÇÃO

02/12/2019

RESUMO

RESUMO Objetivo Avaliar a influência da suplementação de β-hidroxi-β-metilbutirato sobre as características morfológicas e morfométricas do músculo diafragma de ratos e se existe diferenças entre os sexos. Métodos Estudo experimental com 48 ratos Wistar (24 de cada sexo) divididos em 3 grupos: Grupo Controle, no qual foi ofertada dieta diária com soro fisiológico; Grupo Experimental, ofertada 0,3g/kg de β-hidroxi-β-metilbutirato e mesma quantidade de alimentos do Grupo Controle consumida no dia anterior; Grupo Experimental Ad libitum com mesma dose do suplemento, porém com alimentação Ad libitum. A análise foi feita através da histomorfometria e classificação em faixas de área das fibras musculares após 30 dias do início do experimento. Os dados foram analisados pelos testes Anova one-way e Tukey (p<0,05). Resultados Não foi observado aumento da área de secção transversal das fibras do músculo diafragma com o protocolo de suplementação utilizado e no padrão histológico em ambos os sexos. Não foram observadas mudanças significativas nas faixas de tamanho de fibras musculares nos grupos suplementados, sugerindo que não houve hipertrofia das fibras musculares. Conclusão A suplementação com β-hidroxi-β-metilbutirato não provoca alterações nas características morfológicas e morfo-métricas do músculo diafragma, independente do sexo.ABSTRACT Objective To evaluate the β-hydroxy-βmethylbutyrate supplementation influence on the morphological and morphometric characteristics of the diaphragm muscle of rats and verify whether there are sex differences. Methods Experimental study with 48 Wistar rats (24 of each sex) divided into 3 groups: Control Group: in which a daily diet with saline solution was offered; Experimental Group: the same amount of food from the Control group consumed in the previous day and 0.3g/kg of β-hydroxy-βmethylbutyrate; Ad libitum Experimental Group: ad libitum feeding with the same dose of the supplement. The analysis consisted of histomorphometry and classification in diaphragm muscle fiber area bands. The procedures occurred 30 days after the start of the experiment. Data were analyzed using the one-way ANOVA and Tukey tests (p<0.05). Results There was no increase in the cross-sectional area of the diaphragm muscle fibers with the supplementation protocol employed and a similar histological pattern in both sexes. No significant changes were observed in muscle fiber size ranges in the supplemented groups, suggesting that there was no hypertrophy of muscle fibers. Conclusion This study suggests that β-hydroxy-βmethylbutyrate supplementation does not cause changes in the morphological and morphometric characteristics of the diaphragm muscle, regardless of sex.

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