The yggH Gene of Escherichia coli Encodes a tRNA (m7G46) Methyltransferase

AUTOR(ES)

De Bie, Lara G. S.

FONTE

American Society for Microbiology

RESUMO

We cloned, expressed, and purified the Escherichia coli YggH protein and show that it catalyzes the S-adenosyl-l-methionine-dependent formation of N7-methylguanosine at position 46 (m7G46) in tRNA. Additionally, we generated an E. coli strain with a disrupted yggH gene and show that the mutant strain lacks tRNA (m7G46) methyltransferase activity.

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