The vertebrate protein CTCF functions as an insulator in Saccharomyces cerevisiae

AUTOR(ES)

Defossez, Pierre-Antoine

FONTE

Oxford University Press

RESUMO

Insulators are elements that shelter genes from the effects of silencers or enhancers. CTCF is the only vertebrate protein that has a recognized role in transcriptional insulation, but how it exerts its effect is unknown. In an attempt to better understand how CTCF functions, we have used an insulation assay in Saccharomyces cerevisiae. We show that CTCF acts as an insulator in yeast, where it can efficiently block the spreading of repressive telomeric chromatin. We identify two domains of the protein that are responsible for this activity: a short and very potent N-terminal domain, as well as the C-terminus of the protein.

Documentos Relacionados

• Transcriptional repression by the insulator protein CTCF involves histone deacetylases
• Lysis protein S of phage lambda functions in Saccharomyces cerevisiae.
• Bent DNA functions as a replication enhancer in Saccharomyces cerevisiae.
• The Binding Sites for the Chromatin Insulator Protein CTCF Map to DNA Methylation-Free Domains Genome-Wide
• Conserved CTCF Insulator Elements Flank the Mouse and Human β-Globin Loci