The sv40 transcription complex. I. Effect of viral chromatin proteins on endogenous RNA polymerase activity.

AUTOR(ES)

Brooks, T L

RESUMO

SV40 chromatin obtained from infected monkey cells was used to study the effect of the viral chromatin proteins on endogenous RNA polymerase II. Ammonium sulfate activated the rate of transcription by endogenous RNA polymerase in two ways: 1) by direct action on the enzyme; and 2) by causing a reversible conformational change in the viral chromatin. Under optimal reaction conditions, the viral chromatin proteins did not limit the rate of RNA chain elongation, and high molecular weight RNA (1.6 X 10(6) d) was synthesized by the SV40 chromatin.

Documentos Relacionados

• The sv40 transcription complex. II. Non-dissociation of protein from SV40 chromatin during transcription.
• Replicative activity of histone-deficient SV40 chromatin.
• Interaction of the DNA untwisting enzyme with the SV40 nucleoprotein complex.
• The sequence motifs that are involved in SV40 enhancer function also control SV40 late promoter activity.
• RNA polymerase II terminates transcription in vitro in the SV40 origin region.