The Survival of Mutants at Very Low Frequencies in Tribolium Populations

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Forty population cages, each with 499 adult T. castaneum of the wild-type UPF strain, received a bb female newly mated with UPF males. Half of the immigrants had a Chicago Black genetic background, the other half a UPF background. These conditions simulate, respectively, the fate of a rare, genetically differing immigrant or the fate of a mutation in populations of considerable size. Adults were censused for 11 discrete generations. The semi-dominant autosomal black gene survived in 26 out of 40 cultures by the end of the experiment, demonstrating its selective advantage at these very low frequencies. The gene increased from an initial frequency of 0.002 to 0.055 (at generation 11) in at least one replicate. Although frequency-dependent fitness has been shown for black at higher frequencies, no such dependence could be demonstrated at the low frequencies of this study. The cultures simulating mutations (immigrants with native backgrounds) had a higher average gene frequency, different distribution of gene frequencies across replicates, and a lower extinction rate of black than did the cultures with alien background immigrants. The observations only partially fitted expectation based on a branching process model. The data show a tendency for the persistence of a few heterozygotes in cultures and for a deficiency of cultures that lost the mutant or those with many heterozygotes. The increase in frequency of black cannot be attributed to increased reproductive success of heterozygotes. The advantage of heterozygotes appears due to delayed developmental period as a result of tactile stimulation and probable differential cannibalism among pupae.

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