The sum1-1 mutation affects silent mating-type gene transcription in Saccharomyces cerevisiae.
AUTOR(ES)
Livi, G P
RESUMO
The silent mating-type genes (HML and HMR) of Saccharomyces cerevisiae are kept under negative transcriptional control by the trans-acting products of the four MAR/SIR loci. MAR/SIR gene mutations result in the simultaneous derepression of HML and HMR gene expression. The sum1-1 mutation was previously identified as an extragenic suppressor of mutations in MAR1 (SIR2) and MAR2 (SIR3). As assayed genetically, sum1-1 is capable of restoring repression of silent mating-type information in cells containing mar1 or mar2 null mutations. We show here that the mating-type phenotype associated with sum1-1 results from a dramatic reduction in the steady-state level of HML and HMR gene transcripts. At the same time, the sum1-1 mutation has no significant effect on the level of each of the four MAR/SIR mRNAs.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=360769Documentos Relacionados
- A role for CDC7 in repression of transcription at the silent mating-type locus HMR in Saccharomyces cerevisiae.
- A Mutation Allowing Expression of Normally Silent a Mating-Type Information in SACCHAROMYCES CEREVISIAE
- SUM1, an Apparent Positive Regulator of the Cryptic Mating-Type Loci in SACCHAROMYCES CEREVISIAE
- A Mutation That Permits the Expression of Normally Silent Copies of Mating-Type Information in SACCHAROMYCES CEREVISIAE
- Heteroduplex formation and mismatch repair of the "stuck" mutation during mating-type switching in Saccharomyces cerevisiae.
