The skeletal muscle Ca2+ release channel has an oxidoreductase-like domain

AUTOR(ES)

Baker, Matthew L.

FONTE

National Academy of Sciences

RESUMO

We used a combination of bioinformatics, electron cryomicroscopy, and biochemical techniques to identify an oxidoreductase-like domain in the skeletal muscle Ca2+ release channel protein (RyR1). The initial prediction was derived from sequence-based fold recognition for the N-terminal region (41–420) of RyR1. The putative domain was computationally localized to the clamp domain in the cytoplasmic region of a 22Å structure of RyR1. This localization was subsequently confirmed by difference imaging with a sequence specific antibody. Consistent with the prediction of an oxidoreductase domain, RyR1 binds [3H]NAD+, supporting a model in which RyR1 has a oxidoreductase-like domain that could function as a type of redox sensor.

Documentos Relacionados

• Sarcoplasmic reticulum lumenal Ca2+ has access to cytosolic activation and inactivation sites of skeletal muscle Ca2+ release channel.
• Single-channel properties of the recombinant skeletal muscle Ca2+ release channel (ryanodine receptor).
• Calmodulin activation and inhibition of skeletal muscle Ca2+ release channel (ryanodine receptor).
• Topology of the Ca2+ release channel of skeletal muscle sarcoplasmic reticulum (RyR1)
• Activation of the Ca2+ release channel of skeletal muscle sarcoplasmic reticulum by palmitoyl carnitine.