The role of HSP 60 in leucocytes cells of bovine placenta / O papel da HSP 60 nas células leucocitárias da placenta bovina

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The maternal-fetal tolerance involves, besides the immune system, many peculiarities regarding the type of placenta and placentation. The bovine placenta is considered not invasive and, as consequence of a superficial implantation without endometrial invasion, it establishes itself as complex barrier that interposes between the maternal and fetal circulation. In addition to that, placenta is an organ that undergoes continuous differentiation and cellular proliferation besides the high metabolic activity that represents a constant source of stress and challenge for the immune system. As an important component in this context, we highlight the heat shock proteins - HSP, that are usually expressed in physiological conditions but, in situations of stress, their expression increases. In the bovine placenta the expression of HSP 60 and 70 has already been verified; however HSP 60 showed a peculiar expression behavior, with higher staining in the first trimester of pregnancy. Considering the presence and expression profiles of HSP 60 in the bovine placenta allied to the particularity of presenting functional distinct leucocytes populations, a hypothesis was raised regarding the influence of this protein on the placental cells, particularly on the leucocytes in the process of fetal-maternal tolerance. Therefore, this work analyzed the role of HSP 60 in immune mechanisms related to some placental leucocytes by several approaches like proliferation assay by CFSE technique, phagocytosis assay and biochemical assays as lipoperoxidation; cell cycle phases and mitochondrial membrane potential of placental cells in culture. Our results showed that HSP 60 does not influence the proliferation of lymphocytes or any other placental cells in various conditions tested. On the other hand, HSP 60 increases phagocytosis and apoptosis in the third trimester, as well as the production of polyunsaturated oxide radicals and the mitochondrial membrane potential. In view of these results, we may infer that HSP 60 may be modulating the kinetics of activation mechanisms for signaling programmed cellular death between placental and immune cells. This hypothesis assures that, on the termed conceptus, the hormonal variation and the immune tolerance may be responsible for regulating the parturition process.

ASSUNTO(S)

bovinos placenta proliferação placenta proliferation bovine fagocitose hsp hsp phagocytosis

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