The role of diet, transforming growth factor alpha and epidermal growth factor receptor in the cell proliferation and differentiation during the postnatal development of the gastric epithelium of rats. / O papel do alimento, do fator de crescimento transformante alfa e do receptor do fator de crescimento epidermal na proliferação e diferenciação celular durante o desenvolvimento pós-natal do epitélio gástrico de ratos.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Early weaning (EW) causes changes in the gastric mucosa, including the increase in cell proliferation and Transforming Growth Factor (TGFa). In the present study, we evaluated the role of this peptide and its receptor EGFR in the control of the gastric growth. 15-d-old rats were divided into two groups: suckling (control) and EW, in which the pups were separated from the dam and fed with powdered chow. EW increased the number of EGFR-positive cells, accelerated the differentiation of mucous neck cells and augmented the expression of mucin 6. EGFR inhibition with AG1478 decreased cell proliferation and the number of mucous neck cells. Among the proteins involved on EGFR signaling pathways and cell cycle, we found that EW increased the levels of p-ERK1/2 and p-Src, but did not change p-Akt, p21 and p27. We suggest that the diet pattern influences proliferation and differentiation in the gastric epithelium, and the TGFa/EGFR can regulate these processes throughout the postnatal development, probably by activating MAPK and Src signaling pathways.

ASSUNTO(S)

diferenciação celular histologia transforming growth factors alpha proliferação celular receptores do fator de crescimento epidermal fatores de crescimento transformantes alfa estômago de animal rats epidermal growth factors receptors histology cell proliferaration cell differentiation animal stomach ratos

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