The relationship between pyrimidine dimers and replicating DNA in UV-irradiated human fibroblasts.
AUTOR(ES)
Lehmann, A R
RESUMO
The relationship between pyrimidine dimers (measured as endonuclease-sensitive sites) and newly-synthesized DNA has been examined in several different ways, with the following results:- 1. After UV-irradiation of normal human fibroblasts the frequency of pyrimidine dimer sites in sections of DNA which have been synthesized immediately before the UV-irradiation is similar to that in the bulk DNA. 2. The frequency of pyrimidine dimer sites in the parental strands of replicating DNA in UV-irradiated normal human fibroblasts is similar to that in the bulk DNA. 3. In UV-irradiated XP variant cells the size of DNA synthesized in the presence of caffeine immediately after UV irradiation accurately corresponds with the average interdimer distance in the parental DNA. This suggests that in this experimental situation each pyrimidine dimer gives rise to a disocntinuity or a termination site in the daughter strand.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=342355Documentos Relacionados
- Pyrimidine dimers in DNA initiate systemic immunosuppression in UV-irradiated mice.
- Pyrimidine dimers in UV-irradiated poly dI:dC.
- Replication of UV-irradiated DNA in human cell extracts: evidence for mutagenic bypass of pyrimidine dimers.
- Dihydrothymine from UV-irradiated DNA.
- Effects of antipain (a protease inhibitor) on respiration, viability, and excision of pyrimidine dimers in UV-irradiated Escherichia coli cells.
