The RCP–Rab11 Complex Regulates Endocytic Protein SortingD⃞
AUTOR(ES)
Peden, Andrew A.
FONTE
The American Society for Cell Biology
RESUMO
Rab 11 GTPase is an important regulator of endocytic membrane traffic. Recently, we and others have identified a novel family of Rab11 binding proteins, known as Rab11-family interacting proteins (FIPs). One of the family members, Rab coupling protein (RCP), was identified as a protein binding to both Rab4 and Rab11 GTPases. RCP was therefore suggested to serve a dual function as Rab4 and Rab11 binding protein. In this study, we characterized the cellular functions of RCP and mapped its interactions with Rab4 and Rab11. Our data show that RCP interacts only weakly with Rab4 in vitro and does not play the role of coupling Rab11 and Rab4 in vivo. Furthermore, our data indicate that the RCP–Rab11 complex regulates the sorting of transferrin receptors from the degradative to the recycling pathway. We therefore propose that RCP functions primarily as a Rab11 binding protein that regulates protein sorting in tubular endosomes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=491816Documentos Relacionados
- The FIP3-Rab11 Protein Complex Regulates Recycling Endosome Targeting to the Cleavage Furrow during Late CytokinesisD⃞V⃞
- The Rab5 Effector Rabankyrin-5 Regulates and Coordinates Different Endocytic Mechanisms
- Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein
- c-Cbl/Sli-1 regulates endocytic sorting and ubiquitination of the epidermal growth factor receptor
- Rab7 Regulates Late Endocytic Trafficking Downstream of Multivesicular Body Biogenesis and Cargo Sequestration*