The N-terminal 33 amino acid domain of Siva-1 is sufficient for nuclear localization

AUTOR(ES)

Chen, J.Y., Yang, L.X., Huang, Z.F.

FONTE

Braz J Med Biol Res

DATA DE PUBLICAÇÃO

02/12/2013

RESUMO

Siva-1 induces apoptosis in multiple pathological processes and plays an important role in the suppression of tumor metastasis, protein degradation, and other functions. Although many studies have demonstrated that Siva-1 functions in the cytoplasm, a few have found that Siva-1 can relocate to the nucleus. In this study, we found that the first 33 amino acid residues of Siva-1 are required for its nuclear localization. Further study demonstrated that the green fluorescent protein can be imported into the nucleus after fusion with these 33 amino acid residues. Other Siva-1 regions and domains showed less effect on Siva-1 nuclear localization. By site-mutagenesis of all of these 33 amino acid residues, we found that mutants of the first 1-18 amino acids affected Siva-1 nuclear compartmentalization but could not complete this localization independently. In summary, we demonstrated that the N-terminal 33 amino acid residues were sufficient for Siva-1 nuclear localization, but the mechanism of this translocation needs additional investigation.

Documentos Relacionados

• Requirement for acidic amino acid residues immediately N-terminal to the conserved domain of Saccharomyces cerevisiae TFIID.
• The N-Terminal Amino-Acid Sequence of Bovine Proparathyroid Hormone
• Adenovirus Proteins II. N-Terminal Amino Acid Analysis
• The N-terminal adenosine triphosphate binding domain of Hsp90 is necessary and sufficient for interaction with estrogen receptor
• The RAG1 N-terminal domain is an E3 ubiquitin ligase