The lemA gene required for pathogenicity of Pseudomonas syringae pv. syringae on bean is a member of a family of two-component regulators.
AUTOR(ES)
Hrabak, E M
RESUMO
The lemA gene of the plant pathogen Pseudomonas syringae pv. syringae is required for disease lesion formation on bean plants. Cosmid clones that complemented a lemA mutant in trans were isolated previously. The lemA gene was localized by subcloning and transposon mutagenesis. The lemA region and flanking DNA were sequenced, and an open reading frame of 2.7 kb was identified. The nucleotide and predicted amino acid sequences of the lemA gene showed sequence similarity to a family of prokaryotic two-component regulatory proteins. Unlike most of the previously described two-component systems, the lemA gene product contained homology to both components in one protein. Mutations introduced upstream and downstream of the lemA gene failed to locate a gene for a second protein component but identified the putative cysM gene of P. syringae pv. syringae. The cysM gene was located upstream of the lemA gene and was divergently transcribed. The lemA gene product was expressed at low levels in P. syringae pv. syringae and appeared to be positively auto-regulated.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=205956Documentos Relacionados
- Regulation of tabtoxin production by the lemA gene in Pseudomonas syringae.
- A two-component regulatory system required for copper-inducible expression of the copper resistance operon of Pseudomonas syringae.
- Pathovar-specific requirement for the Pseudomonas syringae lemA gene in disease lesion formation.
- Gene Cluster of Pseudomonas syringae pv. “phaseolicola” Controls Pathogenicity of Bean Plants and Hypersensitivity on Nonhost Plants
- Pseudomonas aeruginosa AlgB, a two-component response regulator of the NtrC family, is required for algD transcription.