The Isolation and Characterization of a New Iron-Sulfur Protein from Photosynthetic Membranes

AUTOR(ES)

Malkin, Richard

RESUMO

A new iron-sulfur protein, distinct from the soluble chloroplast ferredoxin, was isolated from chloroplast membranes. The isolated protein, purified to homogeneity, had a molecular weight of about 8000 and 4 atoms of iron and 4 inorganic sulfides per mole. Its absorption spectrum had a broad absorbance band in the 400 nm region, a shoulder at approximately 310 nm, and a peak around 280 nm. The absorbance ratio A400 to A280 was 0.55. The electron paramagnetic resonance spectrum (measured at 12°K) of the reduced protein was similar to that of other reduced iron-sulfur proteins, showing a major resonance line at g = 1.94.

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