The FIS protein binds and bends the origin of chromosomal DNA replication, oriC, of Escherichia coli.

AUTOR(ES)

Gille, H

RESUMO

The FIS protein (factor for inversion stimulation) is known to stimulate site-specific recombination processes, such as the inversion of the G segment of bacteriophage Mu, by binding to specific enhancer sequences. It has also been shown to activate transcription from rRNA promoters both in vitro and in vivo. We have identified a specific binding site for FIS in the center of the origin of chromosomal DNA replication, oriC. The DNA bends upon FIS binding. Occupation of the FIS site and binding of DnaA, the initiator protein, to its adjacent binding site (R3) are mutually exclusive. A fis mutant strain can not be efficiently transformed with plasmids which carry and replicate from oriC, suggesting that FIS is required for minichromosome replication.

Documentos Relacionados

• Transcripts within the replication origin, oriC, of Escherichia coli.
• The FIS protein fails to block the binding of DnaA protein to oriC, the Escherichia coli chromosomal origin.
• Start sites for bidirectional in vitro DNA replication inside the replication origin, oriC, of Escherichia coli.
• The structure of the initiation complex at the replication origin, oriC, of Escherichia coli.
• The origin of replication, oriC, and the dnaA protein are dispensable in stable DNA replication (sdrA) mutants of Escherichia coli K-12.