The Effects on Rabbits of Immunization with Bovine Thyroid-Stimulating Hormone and its Subunits
AUTOR(ES)
Beall, Gildon N.
RESUMO
Rabbits were immunized with bovine thyroid-stimulating hormone (bTSH), bovine Inteinizing hormone (bLH), and their subunits. In two immunization experiments, thyroid-stimulating activity was found in the serum of 6 out of 12 rabbits immunized with bTSHβ subunits. The thyroid-stimulating activity in the anti-bTSHβ sera was greater at 2 h than at 8, was eluted with the globulin fraction from Sephadex G-100, was completely neutralized by both anti-bTSH and anti-rabbit gamma globulin, and was completely suppressed by administration of triiodothyronine (T3) to the immunized rabbit. These findings led to the conclusion that the thyroid-stimulating activity resided in soluble complexes of rabbit TSH bound to anti-bTSHβ. Two of nine rabbits immunized with bTSH developed thyroid-stimulating activity in their serum, but it was nonsuppressible by T3. None of the animals immunized with bTSHα, bLH, bLHβ, or bLHα developed serum thyroid-stimulating activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=302572Documentos Relacionados
- Rabbits immunized with thyroid-stimulating hormone produce autoantiidiotypic thyroid-stimulating antibodies.
- Neutralizing and Non-neutralizing Antibodies to Bovine Thyroid-Stimulating Hormone and its Subunits
- Thyrostimulin, a heterodimer of two new human glycoprotein hormone subunits, activates the thyroid-stimulating hormone receptor
- Effects of thyroid-stimulating hormone on adenyl cyclase activity and intermediary metabolism of “cold” thyroid nodules and normal human thyroid tissue
- Thyroid-Stimulating Activity and Chorionic Gonadotropin
