The effect of carbon dioxide pneumoperitoneum on the healing colonic anastomosis in rats
AUTOR(ES)
Durães, Leonardo de Castro, Farias, Igor Eduardo Caetano de, Morais, Pedro Henrique Alves de, Oliveira, Paulo Gonçalves de, Cavalcanti Neto, Florêncio Figueiredo, Lino Junior, Ruy Souza, Feres, Omar, Sousa, João Batista de
FONTE
Acta Cir. Bras.
DATA DE PUBLICAÇÃO
2013-09
RESUMO
PURPOSE: To investigate the effects of pneumoperitoneum on colonic anastomosis healing. METHODS: Colonic anastomosis was performed in 120 rats divided into four groups: Group I - pneumoperitoneum before laparotomy, Group II - pneumoperitoneum after laparorrhaphy, Group III - pneumoperitoneum before laparotomy and after laparorrhaphy, Group IV - no pneumoperitoneum (control group). Pneumoperitoneum pressure was 5 mmHg. Animals were killed on the 3rd, 7th and 14th postoperative day. Hhistopathological features, anastomosis breaking strength, collagen histomorphometry and hydroxyproline concentration were assessed. RESULTS: Breaking strength between groups: (day 3, p=0.165; day 7, p=0.219; day 14, p=0.539). Histopathology revealed that group II had, on day 7, less infiltration of mononuclear cells (p=0.006), greater infiltration of polymorphonuclear cells (p=0.001) and greater necrosis (p=0.001); and on day 14, less fibrosis. Histomorphometry revealed a decrease in collagen in groups I and III (p<0.001) on day 7 and an increase in groups I and II on day 14 (p<0.001). Hydroxyproline concentration was similar for groups on days 3 (p=0.152), 7 (p=0.913) or 14 (p=0.981). CONCLUSION: Carbon dioxide does not impair the healing of colonic anastomosis in rats.
Documentos Relacionados
- The effects of ghrelin on colonic anastomosis healing in rats
- Effects of the histamine H2 receptor antagonist famotidine on the healing of colonic anastomosis in rats
- Effects of pneumoperitoneum with carbon dioxide and helium on renal function and morphology in rats
- Effects of perioperative hypothermia and reactive oxygen species in the healing of colonic anastomosis in rats
- Effects of carbon dioxide therapy on the healing of acute skin wounds induced on the back of rats