The diagnostic value of circulating microRNAs for middle-aged (40–60-year-old) coronary artery disease patients
AUTOR(ES)
Sayed, Ali Sheikh Md, Xia, Ke, Li, Fei, Deng, Xu, Salma, Umme, Li, Tingbo, Deng, Hai, Yang, Dafeng, Haoyang, Zhou, Yang, TianLun, Peng, Jun
FONTE
Clinics
DATA DE PUBLICAÇÃO
2015-04
RESUMO
OBJECTIVE: Circulating microRNAs have been recognized as promising biomarkers for various diseases. The present study aimed to explore the potential roles of circulating miR-149, miR-424 and miR-765 as non-invasive biomarkers for the diagnosis of coronary artery disease in middle-aged (40–60-year-old) patients. METHODS: Sixty-five stable coronary artery disease patients (49–57 years old), 30 unstable coronary artery disease patients (49–58 years old), and 32 non-coronary artery disease patients (49–-57 years old) who were matched for age, sex, smoking habits, hypertension and diabetes were enrolled in this study. Total RNA was isolated from plasma with TRIzol reagent. Circulating miRNA levels were measured by quantitative real-time polymerase chain reaction. RESULTS: Circulating miR-149 levels were decreased 4.49-fold in stable coronary artery disease patients (1.18 ± 0.84) and 5.09-fold in unstable coronary artery disease patients (1.04 ± 0.65) compared with non-coronary artery disease patients (5.30 ± 2.57) (p<0.001). Circulating miR-424 levels were reduced 3.6-fold in stable coronary artery disease patients (1.18 ± 0.60) and 5-fold in unstable coronary artery disease patients (0.86 ± 0.54) compared with non-coronary artery disease patients (4.35 ± 2.20) (p<0.001). In contrast, circulating miR-765 levels were elevated 3.98-fold in stable coronary artery disease patients (6.09 ± 2.27) and 5.33-fold in unstable coronary artery disease patients (8.17 ± 2.77) compared with non-coronary artery disease patients (1.53 ± 0.99) (p<0.001). Receiver operating characteristic curve analysis revealed that the respective areas under the curve for circulating miR-149, miR-424 and miR-765 were 0.938, 0.919 and 0.968 in stable CAD patients and 0.951, 0.960 and 0.977 in unstable coronary artery disease patients compared with non-coronary artery disease patients. CONCLUSION: Our results suggest that circulating miR-149, miR-424 and miR-765 might be novel, non-invasive biomarkers for the diagnosis of coronary artery disease in middle-aged patients. However, future prospective trials in large patient cohorts are necessary before reaching a solid conclusion.
Documentos Relacionados
- Invasive Coronary Physiological Assessment in Symptomatic Middle-Aged Endurance Athletes
- Origin of the left anterior descending coronary artery from the pulmonary artery: An unusual cause of angina in a middle-aged woman
- Prospective relations between Helicobacter pylori infection, coronary heart disease and stroke in middle-aged men
- A prospective study of the development of coronary heart disease in a group of 1202 middle-aged men.
- Comparing the diagnostic values of circulating microRNAs and cardiac troponin T in patients with acute myocardial infarction