The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5

AUTOR(ES)

Lima, Francisco Esmaile de Sales, Batista, Helena Beatriz Ruthner, Campos, Fabrício Souza, Kunert Filho, Hiran Castagnino, Cibulski, Samuel, Finoketti, Fernando, Arantes, Thalita, Spilki, Fernando, Roehe, Paulo Michel, Rijsewijk, Franciscus Antonius Maria, Franco, Ana Cláudia

FONTE

Braz. arch. biol. technol.

DATA DE PUBLICAÇÃO

2014-02

RESUMO

This study aimed to analyze the effect of the expression of Parainfluenza virus 5 (PIV5) V protein in bovine cells on the replication of Bovine herpesvirus 5 (BoHV-5). Growth properties of BoHV-5 were evaluated in parental and PIV5 transfected cells. In one-step growth experiments, the BoHV-5 reached higher titers at earlier time points in the transfected cells when compared to the parental cells. The mean plaque size produced by the BoHV-5 in transfected cells was larger than the parental cells. This indicated that the expression of the PIV5 V gene facilitated the release and cell-to-cell spread of BoHV-5 in bovine cells.

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