The cloned rRNA genes of P. lophurae: a novel rDNA structure.
AUTOR(ES)
Unnasch, T R
RESUMO
The detailed structure of two ribosomal DNA (rDNA) clones CL-1 and HA-2, from the avian malaria parasite Plasmodium lophurae has been examined using hybridization and electron microscopy. The results demonstrate that the clone CL-1 contains two regions homologous to 25s rRNA of approximately 2200 base pairs (bp) and 450 bp in length, separated by a non homologous region of 240 bp. CL-1 also contains two regions of approximately 1100 bp and 550 bp homologous to 17s rRNA, separated by a non homologous region of 230 bp. The clone HA-2 contains a single region of 670 bp, which is homologous to 25s rRNA. This region is flanked by non homologous stretches of DNA 940 bp and 110 bp in length. As HA-2 is known to be adjacent to CL-1 in the genome (1), these results suggest that the 25s rDNA is interrupted twice, and the 17s rDNA once, by stretches of DNA not found in mature rRNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=326595Documentos Relacionados
- The rDNA of C. elegans: sequence and structure.
- DNA sequence of the 16S rRNA/23S rRNA intercistronic spacer of two rDNA operons of the archaebacterium Methanococcus vannielii.
- Intergenic 16S rRNA gene (rDNA)-23S rDNA sequence length polymorphisms in members of the family Legionellaceae.
- Glucocorticoid inhibition of initiation of transcription of the DNA encoding rRNA (rDNA) in lymphosarcoma P1798 cells.
- The rRNA operon from Zea mays chloroplasts: nucleotide sequence of 23S rDNA and its homology with E.coli 23S rDNA.