The Albino-Deletion Complex of the Mouse: Molecular Mapping of Deletion Breakpoints That Define Regions Necessary for Development of the Embryonic and Extraembryonic Ectoderm
AUTOR(ES)
Sharan, S. K.
RESUMO
Previous complementation analyses with five (c(11DSD), c(5FR60Hg), c(2YPSj), c(4FR60Hd), c(6H)) of the mouse albino deletions defined at least two genes on chromosome 7, known as eed and exed, which are necessary for development of the embryonic and extraembryonic ectoderm, respectively, of early postimplantation embryos. The region of chromosome 7 containing these two genes has now been accessed at the molecular level by cloning two of the deletion breakpoint-fusion fragments. The c(2YPSj) breakpoints were isolated by cloning an EcoRI fragment containing a copy of an albino region-specific repeat unique to c(2YPSj) DNA. Similarly, the c(11DSD) breakpoints were isolated by cloning a c(11DSD) EcoRI fragment detected by a unique-sequence probe mapping proximal to the albino-coat-color locus. By mapping the cloned breakpoints relative to the remaining three deletions, the c(11DSD) distal breakpoint was found to define the distal limit of the region containing eed, whereas the c(2YPSj) and c(6H) distal breakpoints were found to define the proximal and distal limits, respectively, of the region containing exed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1204749Documentos Relacionados
- Molecular mapping within the mouse albino-deletion complex.
- Murine Albino-Deletion Complex: High-Resolution Microsatellite Map and Genetically Anchored Yac Framework Map
- Analysis of the Albino-Locus Region of the Mouse: IV. Characterization of 34 Deficiencies
- Metaxin, a gene contiguous to both thrombospondin 3 and glucocerebrosidase, is required for embryonic development in the mouse: implications for Gaucher disease.
- Deletion mutants of herpesvirus saimiri define an open reading frame necessary for transformation.
