Technique for Measuring 14CO2 Uptake by Soil Microorganisms In Situ
AUTOR(ES)
Smith, David W.
RESUMO
Uptake of 14CO2 in soils due to algae or sulfur-oxidizing bacteria was examined by incubation of soil samples with gaseous 14CO2 and subsequent chemical oxidation of biologically fixed radioactive isotope to 14CO2 for detection with a liquid scintillation counting system. The 14CO2 was added to the soil in the gas phase so that no alteration of the moisture or ionic strength of the soil occurred. Wet oxidation of radioactive organic matter was carried out in sealed ampoules, and the 14CO2 produced was transferred to a phenethylamine-liquid scintillation counting system with a simply constructed apparatus. The technique is inexpensive and efficient and does not require elaborate traps since several possible interfering factors were found to have no harmful effects. Experiments in coal mine regions and in geothermal habitats have demonstrated the ecological applicability of this technique for measurement of CO2 fixation by sulfur-oxidizing bacteria and soil algae.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=380393Documentos Relacionados
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