Systemic infection of mice by wild-type but not Spv- Salmonella typhimurium is enhanced by neutralization of gamma interferon and tumor necrosis factor alpha.
AUTOR(ES)
Gulig, P A
RESUMO
The spv genes of the virulence plasmid of Salmonella typhimurium and other nontyphoidal serovars of S. enterica are involved in systemic infection by increasing the replication rate of the bacteria in host tissues beyond the intestines. We considered the possibility that the Spv virulence function is to evade suppression by the host response to infection. To examine this possibility, gamma interferon (IFN-gamma) and/or tumor necrosis factor alpha (TNF-alpha) were neutralized in BALB/c mice by intraperitoneal administration of monoclonal antibodies. Neutralization of IFN-gamma and/or TNF-alpha resulted in increased splenic infection with wild-type salmonellae after oral inoculation; however, Spv- salmonellae were defective at increasing splenic infection in cytokine-depleted mice. The use of a temperature-sensitive marker plasmid, pHSG422, indicated that neutralization of IFN-gamma caused less killing of wild-type S. typhimurium, while neutralization of TNF-alpha resulted in an increased in vivo replication rate for wild-type salmonellae. These results demonstrate that the Spv virulence function is not to evade suppression of bacterial infection normally mediated by IFN-gamma or TNF-alpha.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=175748Documentos Relacionados
- Role of gamma interferon and tumor necrosis factor alpha in resistance to Salmonella typhimurium infection.
- Utilization of D-xylose by wild-type strains of Salmonella typhimurium.
- Protection of mice against Listeria monocytogenes infection by recombinant human tumor necrosis factor alpha.
- Wild-type isopropylmalate isomerase in Salmonella typhimurium is composed of two different subunits.
- Association between virulence of Yersinia pestis and suppression of gamma interferon and tumor necrosis factor alpha.
