Synthesis and properties of DNA-PNA chimeric oligomers.
AUTOR(ES)
Finn, P J
RESUMO
Adenine, thymine and cytosine PNA monomers have been prepared using 3-amino-1,2-propanediol as a starting material. The benzoyl group was used to protect the exocyclic amines of the heterocyclic bases of A and C PNA monomers and the backbone primary amine was protected with the monomethoxytrityl group. The thymine and cytosine PNA monomers were used in conjunction with standard DNA synthesis monomers to produce chimeric PNA DNA (PDC) oligomers. Ultraviolet melting studies confirmed that these oligomers form stable hybrids with complementary DNA strands and that mismatches in the DNA but more so in the PNA sections lead to duplex destabilisation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=146105Documentos Relacionados
- Synthesis and evaluation of some properties of chimeric oligomers containing PNA and phosphono-PNA residues.
- Prediction of 1H NMR chemical shifts of DNA oligomers.
- An assessment of the antisense properties of RNase H-competent and steric-blocking oligomers.
- Strand-invasion of duplex DNA by peptide nucleic acid oligomers.
- Hydration effects on the duplex stability of phosphoramidate DNA-RNA oligomers.
