Synthesis and modification of proteins during the cell cycle of the yeast Saccharomyces cerevisiae.
AUTOR(ES)
Elliott, S G
RESUMO
We have used a novel technique to study the synthesis, modification and degradation of proteins during the cell cycle in Saccharomyces cerevisiae. Logarithmically growing cells were pulse-labeled twice, with the pulses separated in time by more than one generation. Subsequently, the cells were fractionated as to their position in the cell cycle by centrifugal elutriation, and for different proteins the ratio of radioactive material from the two pulses was then determined. Periodic degradation, synthesis, or modification would produce periodic variations in the ratio of counts. Two-dimensional gel electrophoresis was used to examine 110 different proteins at different times of the cell cycle. All but two proteins had a constant ratio of counts through the cell cycle. This indicates that the rate of synthesis of individual proteins increases exponentially during the cell cycle and that periodic degradation or modification of proteins is not a general feature of the cell cycle in S. cerevisiae.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=218300Documentos Relacionados
- Synthesis of ribosomal proteins during the cell cycle of the yeast Saccharomyces cerevisiae.
- Rate of macromolecular synthesis through the cell cycle of the yeast Saccharomyces cerevisiae.
- Buoyant density variation during the cell cycle of Saccharomyces cerevisiae.
- Cell size and budding during starvation of the yeast Saccharomyces cerevisiae.
- Identification of Proteins Whose Synthesis Is Modulated During the Cell Cycle of Saccharomyces cerevisiae