Synergism of v-myc and v-Ha-ras in the in vitro neoplastic progression of murine lymphoid cells.

AUTOR(ES)

Schwartz, R C

RESUMO

Murine bone marrow was either singly or doubly infected with retroviral vectors expressing v-myc (OK10) or v-Ha-ras. The infected bone marrow was cultured in a system that supports the long-term growth of B-lineage lymphoid cells. While the v-myc vector by itself had no apparent effect on lymphoid culture establishment and growth, infection with the v-Ha-ras vector or coinfection with both v-myc and v-Ha-ras vectors led to the appearance of growth-stimulated cell populations. Clonal pre-B-cell lines stably expressing v-Ha-ras alone or both v-myc and v-Ha-ras grew out of these cultures. In comparison with cell lines expressing v-Ha-ras alone, cell lines expressing both v-myc and v-Ha-ras grew to higher densities, had reduced dependence on a feeder layer for growth, and had a marked increase in ability to grow in soft-agar medium. The cell lines expressing both oncogenes were highly tumorigenic in syngeneic animals. These experiments show that the v-myc oncogene in synergy with v-Ha-ras can play a direct role in the in vitro transformation of murine B lymphoid cells.

Documentos Relacionados

• Coinfection with viruses carrying the v-Ha-ras and v-myc oncogenes leads to growth factor independence by an indirect mechanism.
• Introduction of v-Ha-ras oncogene induces differentiation of cultured human medullary thyroid carcinoma cells.
• v-Ha-ras oncogene insertion: a model for tumor progression of human small cell lung cancer.
• Activated v-myc and v-ras oncogenes do not transform normal human lymphocytes.
• Follicular origin of epidermal papillomas in v-Ha-ras transgenic TG.AC mouse skin.