Synaptophysin regulates activity-dependent synapse formation in cultured hippocampal neurons
AUTOR(ES)
Tarsa, Leila
FONTE
The National Academy of Sciences
RESUMO
Synaptophysin is an abundant synaptic vesicle protein without a definite synaptic function. Here, we examined a role for synaptophysin in synapse formation in mixed genotype micro-island cultures of wild-type and synaptophysin-mutant hippocampal neurons. We show that synaptophysin-mutant synapses are poor donors of presynaptic terminals in the presence of competing wild-type inputs. In homogenotypic cultures, however, mutant neurons display no apparent deficits in synapse formation compared with wild-type neurons. The reduced extent of synaptophysin-mutant synapse formation relative to wild-type synapses in mixed genotype cultures is attenuated by blockers of synaptic transmission. Our findings indicate that synaptophysin plays a previously unsuspected role in regulating activity-dependent synapse formation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=117422Documentos Relacionados
- Activity-dependent current distributions in model neurons.
- Activity-dependent regulation of synaptic clustering in a hippocampal culture system
- Proteolytic action of thrombin is required for electrical activity-dependent synapse reduction.
- Context sensitivity of activity-dependent increases in cerebral blood flow
- Activity-dependent accumulation of calcium in Purkinje cell dendritic spines.