Symmetrical In Vivo Transcription of Mitochondrial DNA in HeLa Cells

AUTOR(ES)

Aloni, Y.

RESUMO

RNA·DNA hybridization experiments utilizing separated strands of HeLa mitochondrial DNA and mit-RNA from HeLa cells exposed to short pulses of [5-3H]uridine have shown that the labeled RNA hybridizes with both the light (L) and the heavy (H) strand, though to a different relative extent depending upon the labeling time. Thus, hybridization of pulse-labeled RNA is about equal with the two strands when the pulse is very short (1-5 min), and becomes more and more predominant with the H strand with increasing pulse length. Pulse-labeled fast-sedimenting mit-RNA forms RNase-resistant double-stranded structures up to more than 5 μm long when self-annealed or annealed with an excess of unlabeled mit-RNA. These observations and the previous evidence of complete transcription of the H strand strongly suggest that mit-DNA is transcribed in HeLa cells symmetrically over a considerable portion of its length, with the transcript of the L strand being rapidly degraded or otherwise removed from the mitochondrial fraction.

Documentos Relacionados

• Modulation of mitochondrial transcription in response to mtDNA depletion and repletion in HeLa cells
• Mitochondrial DNA synthesis in adenovirus type 2-infected HeLa cells.
• Introduction of disease-related mitochondrial DNA deletions into HeLa cells lacking mitochondrial DNA results in mitochondrial dysfunction.
• Mitochondrial origin of membrane-associated heterogeneous RNA in HeLa cells.
• The presence of intact mitochondrial DNA in HeLa cell nuclei.