Susceptibility of chemostat-grown Yersinia enterocolitica and Klebsiella pneumoniae to chlorine dioxide.

AUTOR(ES)

Harakeh, M S

RESUMO

The resistance of bacteria to antimicrobial agents could be influenced by growth environment. The susceptibility of two enteric bacteria, Yersinia enterocolitica and Klebsiella pneumoniae, to chlorine dioxide was investigated. These organisms were grown in a defined medium in a chemostat and the influence of growth rate, temperature, and cell density on the susceptibility was studied. All inactivation experiments were conducted with a dose of 0.25 mg of chlorine dioxide per liter in phosphate-buffered saline at pH 7.0 and 23 degrees C. The results indicated that populations grown under conditions that more closely approximate natural aquatic environments, e.g., low temperatures and growth at submaximal rates caused by nutrient limitation, were most resistant. The conclusion from this study is that antecedent growth conditions have a profound effect on the susceptibility of bacteria to disinfectants, and it is more appropriate to use the chemostat-grown bacteria as test organisms to evaluate the efficacy of a certain disinfectant.

Documentos Relacionados

• Teichoic acids from chemostat-grown cultures of Streptococcus mutans and Lactobacillus plantarum.
• Serological studies on chemostat-grown cultures of Lactobacillus fermentum and Lactobacillus plantarum.
• Growth-rate-dependent adjustment of ribosome function in chemostat-grown cells of the fungus Mucor racemosus.
• Inactivation of human and simian rotaviruses by chlorine dioxide.
• Effect of antecedent growth conditions on sensitivity of Escherichia coli to chlorine dioxide.