Susceptibility and molecular characterization of Candida species from patients with vulvovaginitis
Fornari, Gheniffer, Vicente, Vania Aparecida, Gomes, Renata Rodrigues, Muro, Marisol Dominguez, Pinheiro, Rosangela Lameira, Ferrari, Carolina, Herkert, Patricia Fernanda, Takimura, Marcos, Carvalho, Newton Sérgio de, Queiroz-Telles, Flavio
Braz. J. Microbiol.
DATA DE PUBLICAÇÃO
Abstract Vulvovaginal candidiasis affects women of reproductive age, which represents approximately 15–25% of vaginitis cases. The present study aimed to isolate and characterize yeast from the patients irrespective of the presentation of clinical symptoms. The isolates were subjected to in vitro susceptibility profile and characterization by molecular markers, which intended to assess the distribution of species. A total of 40 isolates were obtained and identified through the CHROMagar, API20aux and by ITS and D1/D2 regions sequencing of DNAr gene. Candida albicans strains were genotyped by the ABC system and the isolates were divided into two genotypic groups. The identity of the C. albicans, C. glabrata, C. guilliermondii, C. kefyr and Saccharomyces cerevisiae isolates was confirmed by the multilocus analysis. The strains of Candida, isolated from patients with complications, were found to be resistant to nystatin but sensitive to fluconazole, amphotericin B and ketoconazole, as observed by in vitro sensitivity profile. The isolates from asymptomatic patients, i.e., the colonized group, showed a dose-dependent sensitivity to the anti-fungal agents, fluconazole and amphotericin B. However, the isolates of C. albicans that belong to distinct genotypic groups showed the same in vitro susceptibility profile.
- Molecular characterization of Candida spp. isolates from patients with bloodstream infections
- Susceptibility Pattern and Molecular Type of Species-Specific Candida in Oropharyngeal Lesions of Indian Human Immunodeficiency Virus-Positive Patients
- Molecular identification and antifungal susceptibility profiles of Candida parapsilosis complex species isolated from culture collection of clinical samples
- Diagnosis of Candida vulvovaginitis.
- Antimicrobial susceptibility of Aeromonas species isolated from patients with diarrhea.