Surface response methodology for the optimization of lipase production under submerged fermentation by filamentous fungi

AUTOR(ES)

Colla, Luciane Maria, Primaz, Andreiza Lazzarotto, Benedetti, Silvia, Loss, Raquel Aparecida, Lima, Marieli de, Reinehr, Christian Oliveira, Bertolin, Telma Elita, Costa, Jorge Alberto Vieira

FONTE

Braz. J. Microbiol.

DATA DE PUBLICAÇÃO

2016-06

RESUMO

Abstract A Plackett–Burman Factorial Design of 16 experiments was conducted to assess the influence of nine factors on the production of lipases by filamentous fungi. The factors investigated were bran type (used as the main carbon source), nitrogen source, nitrogen source concentration, inducer, inducer concentration, fungal strain (Aspergillus niger or Aspergillus flavus were selected as good lipase producers via submerged fermentation), pH and agitation. The concentration of the yeast extract and soybean oil and the pH had a significant effect (p < 0.05) on lipase production and were consecutively studied through a Full Factorial Design 23, with the concentration of yeast extract and pH being significant (p < 0.05). These variables were optimized using a central composite design, obtaining maximum lipolytic activities with the use of 45 g/L of yeast extract and pH 7.15. The statistical model showed a 94.12% correlation with the experimental data.

Documentos Relacionados

• Response surface methodology for optimization of production of lovastatin by solid state fermentation
• Optimization of Lipase-Catalyzed Transesterification of Cotton Seed Oil for Biodiesel Production Using Response Surface Methodology
• Lipase Production by Aspergillus niger C by Submerged Fermentation
• Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenes
• Computation of interactive effects and optimization of process parameters for alkaline lipase production by mutant strain of Pseudomonas aeruginosa using response surface methodology