Suicide plasmid vehicles for insertion mutagenesis in Rhizobium meliloti and related bacteria.
AUTOR(ES)
Selvaraj, G
RESUMO
We describe the construction and use of a set of plasmid vectors of the transposons Tn1, Tn5, and Tn9 that are suicidal in Rhizobium species and therefore suitable for mutagenesis with these three transposons. The vectors are composed of the p15A replicon which functions in Escherichia coli but not in Rhizobium species and a region encoding the N type of bacterial conjugation system which is very efficient in matings between E. coli and Rhizobium species. The usefulness of the vectors has been most extensively assessed in Rhizobium meliloti. It is likely that they will be useful for mutagenesis and genome manipulation in other bacteria as well.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=217980Documentos Relacionados
- Gene fusion vehicles for the analysis of gene expression in Rhizobium meliloti.
- Development and testing of improved suicide functions for biological containment of bacteria.
- Development of efficient suicide mechanisms for biological containment of bacteria.
- Cloning and mutagenesis of the Rhizobium meliloti isocitrate dehydrogenase gene.
- New thermosensitive plasmid for gram-positive bacteria.
